| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Cerrados. |
|
Data corrente: |
23/11/2018 |
|
Data da última atualização: |
29/11/2018 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Autoria: |
PESSOA FILHO, M. A. C. de P.; SOUZA SOBRINHO, F. de; FRAGOSO, R. da R.; SILVA JUNIOR, O. B. da; FERREIRA, M. E. |
|
Afiliação: |
MARCO AURELIO CALDAS DE PINHO PESSO, CPAC; FAUSTO DE SOUZA SOBRINHO, CNPGL; RODRIGO DA ROCHA FRAGOSO, CPAC; ORZENIL BONFIM DA SILVA JUNIOR, Cenargen; MARCIO ELIAS FERREIRA, SIRE. |
|
Título: |
A draft genome assembly for the forage grass Urochloa ruziziensis based on single-molecule real-time sequencing. |
|
Ano de publicação: |
2018 |
|
Fonte/Imprenta: |
In: BRAZILIAN BIOTECHNOLOGY CONGRESS, 7.; BIOTECHNOLOGY IBERO-AMERICAN CONGRESS, 2., 2018, Brasília, DF. Proceedings... Brasília, DF: SBBiotec, 2018. |
|
Idioma: |
Inglês |
|
Conteúdo: |
ABSTRACT: Ruzigrass (Urochloa ruziziensis) is a diploid, tropical forage grass native to Africa, widely planted in Brazil, and known for its high nutritional quality. It is closely related to other important forage species of Urochloa, playing a crucial role in the breeding program of brachiaria grasses, which is mostly focused on inter-specific hybrids. Previous studies from our group based on shallow Illumina sequencing resulted in the development of the first molecular markers for the species, as well as in assessments of germplasm diversity and structure. Assembly and analysis of complete plastid genomes for four Urochloa species allowed the characterization of their phylogenetic divergence. Here, we strengthen the set of genomic tools for tropical forage grasses by assembling long reads into a first draft for the nuclear genome of the heterozygous clone C69. We used PacBio Sequel to generate over 13.3 million raw reads (mean size 6.5 kbp), adding up to 87.5 Gbp of raw data (~142x coverage). The current assembly using FALCON contains ~1.02 Gbp in 7,628 primary contigs, with NG50 of 412 kbp. It covers almost twice the size of the estimated haploid genome size of 615 Mbp for ruzigrass, indicating that haplotypes were assembled separately. In addition, RNA-seq data was obtained, totaling 258 million reads and 26 Gbp of raw data. These will allow transcriptome characterization for different tissues and aid gene prediction and annotation. Ongoing research includes haplotype phasing, polishing, assembly curation and Hi-C scaffolding. A high-quality genome assembly for ruzigrass will aid research groups in the development and application of genomic tools in breeding and genetics of brachiaria grasses. MenosABSTRACT: Ruzigrass (Urochloa ruziziensis) is a diploid, tropical forage grass native to Africa, widely planted in Brazil, and known for its high nutritional quality. It is closely related to other important forage species of Urochloa, playing a crucial role in the breeding program of brachiaria grasses, which is mostly focused on inter-specific hybrids. Previous studies from our group based on shallow Illumina sequencing resulted in the development of the first molecular markers for the species, as well as in assessments of germplasm diversity and structure. Assembly and analysis of complete plastid genomes for four Urochloa species allowed the characterization of their phylogenetic divergence. Here, we strengthen the set of genomic tools for tropical forage grasses by assembling long reads into a first draft for the nuclear genome of the heterozygous clone C69. We used PacBio Sequel to generate over 13.3 million raw reads (mean size 6.5 kbp), adding up to 87.5 Gbp of raw data (~142x coverage). The current assembly using FALCON contains ~1.02 Gbp in 7,628 primary contigs, with NG50 of 412 kbp. It covers almost twice the size of the estimated haploid genome size of 615 Mbp for ruzigrass, indicating that haplotypes were assembled separately. In addition, RNA-seq data was obtained, totaling 258 million reads and 26 Gbp of raw data. These will allow transcriptome characterization for different tissues and aid gene prediction and annotation. Ongoing research includes haplotype p... Mostrar Tudo |
|
Palavras-Chave: |
Bioinformática; De novo Assembly; Montagem de sequência de DNA. |
|
Thesagro: |
Brachiaria Ruziziensis; Genoma; Pastagem. |
|
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/186953/1/920-apagar.pdf
|
|
Marc: |
LEADER 02571nam a2200229 a 4500
001 2099953
005 2018-11-29
008 2018 bl uuuu u00u1 u #d
100 1 $aPESSOA FILHO, M. A. C. de P.
245 $aA draft genome assembly for the forage grass Urochloa ruziziensis based on single-molecule real-time sequencing.$h[electronic resource]
260 $aIn: BRAZILIAN BIOTECHNOLOGY CONGRESS, 7.; BIOTECHNOLOGY IBERO-AMERICAN CONGRESS, 2., 2018, Brasília, DF. Proceedings... Brasília, DF: SBBiotec$c2018
520 $aABSTRACT: Ruzigrass (Urochloa ruziziensis) is a diploid, tropical forage grass native to Africa, widely planted in Brazil, and known for its high nutritional quality. It is closely related to other important forage species of Urochloa, playing a crucial role in the breeding program of brachiaria grasses, which is mostly focused on inter-specific hybrids. Previous studies from our group based on shallow Illumina sequencing resulted in the development of the first molecular markers for the species, as well as in assessments of germplasm diversity and structure. Assembly and analysis of complete plastid genomes for four Urochloa species allowed the characterization of their phylogenetic divergence. Here, we strengthen the set of genomic tools for tropical forage grasses by assembling long reads into a first draft for the nuclear genome of the heterozygous clone C69. We used PacBio Sequel to generate over 13.3 million raw reads (mean size 6.5 kbp), adding up to 87.5 Gbp of raw data (~142x coverage). The current assembly using FALCON contains ~1.02 Gbp in 7,628 primary contigs, with NG50 of 412 kbp. It covers almost twice the size of the estimated haploid genome size of 615 Mbp for ruzigrass, indicating that haplotypes were assembled separately. In addition, RNA-seq data was obtained, totaling 258 million reads and 26 Gbp of raw data. These will allow transcriptome characterization for different tissues and aid gene prediction and annotation. Ongoing research includes haplotype phasing, polishing, assembly curation and Hi-C scaffolding. A high-quality genome assembly for ruzigrass will aid research groups in the development and application of genomic tools in breeding and genetics of brachiaria grasses.
650 $aBrachiaria Ruziziensis
650 $aGenoma
650 $aPastagem
653 $aBioinformática
653 $aDe novo Assembly
653 $aMontagem de sequência de DNA
700 1 $aSOUZA SOBRINHO, F. de
700 1 $aFRAGOSO, R. da R.
700 1 $aSILVA JUNIOR, O. B. da
700 1 $aFERREIRA, M. E.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Cerrados (CPAC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
|
Data corrente: |
30/09/2019 |
|
Data da última atualização: |
30/12/2020 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 1 |
|
Autoria: |
MORANDI, P. S.; MARIMON, B. S.; MARIMON-JUNIOR, B. H.; RATTER, J. A.; FELDPAUSCH, T. R.; COLLI, G. R.; MUNHOZ, C. B. R.; SILVA JÚNIOR, M. C. da; LIMA, E. de S.; HAIDAR, R. F.; ARROYO, L.; MURAKAMI, A. A.; AQUINO, F. de G.; WALTER, B. M. T.; RIBEIRO, J. F.; FRANÇOSO, R.; ELIAS, R.; OLIVEIRA, E. A. de; REIS, S. M.; OLIVEIRA, B. de; NEVES, E. C. das; NOGUEIRA, D. S.; LIMA, H. S.; CARVALHO, T. P. de; RODRIGUES, S. A.; VILLARROEL, D.; FELFILI, J. M.; PHILLIPS, O. L. |
|
Afiliação: |
PAULO S. MORANDI, BIONORTE, UFAM–UNEMAT; BEATRIZ SCHWANTES MARIMON, BIONORTE, UFAM–UNEMAT; BEN HUR MARIMON-JUNIOR, BIONORTE, UFAM–UNEMAT; JAMES A. RATTER, ROYAL BOTANIC GARDEN, UK; TED R. FELDPAUSCH, UNIVERSITY OF EXETER, UK; GUARINO RINALDI COLLI, UNB; CÁSSIA BEATRIZ RODRIGUES MUNHOZ, UNB; MANOEL CLÁUDIO DA SILVA JÚNIOR, UNB; EDSON DE SOUZA LIMA, INSTITUTO PRÓ-CARNÍVOROS; RICARDO FLORES HAIDAR, UNB; LUZMILA ARROYO, UNIVERSIDAD AUTÓNOMA GABRIEL RENE MORENO, BOLIVIA; ALEJANDRO ARAUJO MURAKAMI, UNIVERSIDAD AUTÓNOMA GABRIEL RENE MORENO, BOLIVIA; FABIANA DE GOIS AQUINO, CPAC; BRUNO MACHADO TELES WALTER, Cenargen; JOSE FELIPE RIBEIRO, CPAC; RENATA FRANÇOSO, UNB; FERNANDO ELIAS, UNEMAT; EDMAR ALMEIDA DE OLIVEIRA, BIONORTE, UFAM–UNEMAT; SIMONE MATIAS REIS, BIONORTE, UFAM–UNEMAT; BIANCA DE OLIVEIRA, UNEMAT; EDER CARVALHO DAS NEVES, UNEMAT; DENIS SILVA NOGUEIRA, UNEMAT; HERSON SOUZA LIMA, INSTITUTO PRÓ-CARNÍVOROS; TATIANE PIRES DE CARVALHO, INSTITUTO PRÓ-CARNÍVOROS; SILVO ALVES RODRIGUES, UFMT; DANIEL VILLARROEL, UNIVERSIDAD AUTÓNOMA GABRIEL RENE MORENO, BOLIVIA; JEANINE M. FELFILI, UNB; OLIVER L. PHILLIPS, UNIVERSITY OF LEEDS, UK. |
|
Título: |
Tree diversity and above-ground biomass in the South America Cerrado biome and their conservation implications. |
|
Ano de publicação: |
2020 |
|
Fonte/Imprenta: |
Biodiversity and Conservation, v. 29, n. 5, p. 1519-1536, 2020. |
|
DOI: |
10.1007/s10531-018-1589-8 |
|
Idioma: |
Inglês |
|
Palavras-Chave: |
Carbon stocks; Core area; Diversity-biomass; Neotropics; Richness; Transition. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 01479naa a2200517 a 4500
001 2128856
005 2020-12-30
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1007/s10531-018-1589-8$2DOI
100 1 $aMORANDI, P. S.
245 $aTree diversity and above-ground biomass in the South America Cerrado biome and their conservation implications.$h[electronic resource]
260 $c2020
653 $aCarbon stocks
653 $aCore area
653 $aDiversity-biomass
653 $aNeotropics
653 $aRichness
653 $aTransition
700 1 $aMARIMON, B. S.
700 1 $aMARIMON-JUNIOR, B. H.
700 1 $aRATTER, J. A.
700 1 $aFELDPAUSCH, T. R.
700 1 $aCOLLI, G. R.
700 1 $aMUNHOZ, C. B. R.
700 1 $aSILVA JÚNIOR, M. C. da
700 1 $aLIMA, E. de S.
700 1 $aHAIDAR, R. F.
700 1 $aARROYO, L.
700 1 $aMURAKAMI, A. A.
700 1 $aAQUINO, F. de G.
700 1 $aWALTER, B. M. T.
700 1 $aRIBEIRO, J. F.
700 1 $aFRANÇOSO, R.
700 1 $aELIAS, R.
700 1 $aOLIVEIRA, E. A. de
700 1 $aREIS, S. M.
700 1 $aOLIVEIRA, B. de
700 1 $aNEVES, E. C. das
700 1 $aNOGUEIRA, D. S.
700 1 $aLIMA, H. S.
700 1 $aCARVALHO, T. P. de
700 1 $aRODRIGUES, S. A.
700 1 $aVILLARROEL, D.
700 1 $aFELFILI, J. M.
700 1 $aPHILLIPS, O. L.
773 $tBiodiversity and Conservation$gv. 29, n. 5, p. 1519-1536, 2020.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Cerrados (CPAC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
